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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A patient with a history of recurrent Clostridioides difficile infection (CDI) is being considered for fecal microbiota transplantation (FMT). Pre-FMT screening of the donor stool sample should include comprehensive testing to exclude potential transmission of enteric pathogens. Which of the following would be the MOST critical to rule out in the donor stool?

A) Presence of non-toxigenic C. difficile strains.
B) Evidence of asymptomatic carriage of multidrug-resistant organisms (MDROs) such as vancomycin- resistant enterococci (VRE) or carbapenem-resistant Enterobacterales (CRE).
C) Detection of common antibiotic resistance genes not currently expressed.
D) Quantification of the overall microbial diversity using 16S rRNA gene sequencing.

2. Which pattern on a Kirby-Bauer disk diffusion plate, showing resistance of Staphylococcus aureus to erythromycin but susceptibility to clindamycin with a flattening of the clindamycin zone adjacent to the erythromycin disk (D-zone test positive), indicates inducible clindamycin resistance?

A) Indicates the strain possesses the msrA gene
B) Indicates the strain is tolerant to clindamycin
C) Indicates the strain should be reported as clindamycin susceptible
D) Indicates the strain possesses an erm gene and should be reported as clindamycin resistant

3. A clinical microbiology laboratory is using Sanger sequencing to identify mutations associated with antibiotic resistance in Mycobacterium tuberculosis. This method directly determines the:

A) Presence or absence of specific resistance genes.
B) Relative abundance of different M. tuberculosis strains in a mixed infection.
C) Minimum inhibitory concentration (MIC) of the antibiotic.
D) Nucleotide sequence of the target gene.

4. A patient with a history of solid organ transplantation develops diarrhea and abdominal cramps. Stool studies are negative for bacterial pathogens and C. difficile toxins. Colonoscopy reveals characteristic cytomegalic inclusion bodies in colonic biopsies. The MOST likely causative agent is:

A) Adenovirus
B) Norovirus
C) Rotavirus
D) Cytomegalovirus (CMV)

5. A patient with a history of chronic lung disease develops a severe pneumonia. Bronchoalveolar lavage fluid reveals the presence of numerous cysts containing multiple nuclei, which stain positively with silver stains but poorly with Gram stain. The patient's CD4+ T-cell count is within the normal range. The MOST likely causative organism and the preferred treatment are:

A) Blastomyces dermatitidis; itraconazole.
B) Pneumocystis jirovecii; trimethoprim-sulfamethoxazole.
C) Histoplasma capsulatum; amphotericin B followed by itraconazole.
D) Paragonimus westermani; praziquantel.

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