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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Blood Banking | 15–20% | - Blood products and components - Blood group systems and immunology - Transfusion practice and testing |
| Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
| Laboratory Operations | 5–10% | - Instrumentation and laboratory mathematics - Quality assessment and safety |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Limited to blood and body fluids visibly contaminated with blood
- A. Transmission Precautions
- B. Isolation
- C. Pathogenic
- D. Universal Precautions
Correct Answer: D 🗳️
Entamoeba gingivalis resembles Entamoeba histolytica both in size and in nuclear characteristics. Entamoeba gingivalis may contain numerous cytoplasmic inclusions such as red blood cells, white blood cells, and bacteria.
I reside in the mouth where I measure approximately 17 micro meters.
- A. Entamoeba coli trophozoite
- B. Entamoeba gingivalis trophozoite
- C. Acanthamoeba species trophozoite
- D. Trichomonas vaginalis trophozoite
Correct Answer: B 🗳️
CAMP is the correct answer because the case is typical of Streptococcus agalactiae(Group B strep). The main presumptive identification test is CAMP. Bile solubility is positive for Streptococcus pneumonia. Coagulase would be positive for Staphylococcus aureus. PYR is positive for Streptococcus pyogenes (Group A strep) which is ruled out by the resistance to bacitracin.
Microbiology
Spinal fluid cultures on a 3-day-old infant revealed beta-hemolytic, Gram-positive cocci occurring in pairs and chains. The organism was catalase-negative and bile esculin-negative. Resistance to 0.04 U disk of bacitracin was noted. The other test necessary to identify this organism is a positive test by:
- A. CAMP
- B. Bile solubility
- C. PYR
- D. Coagulase
Correct Answer: A 🗳️
Heinz bodies occur as the result of denaturation and precipitation of hemoglobin, and are often attached to the red cell membrane. They require staining with crystal violet or methyl violet to be visible. They may be seen in thalassemia, with unstable hemoglobins, or during a hemolytic episode in G6PD deficiency.
Hematology
The intracellular precipitates seen in the RBCs in this illustration is termed:
- A. Dohle bodies
- B. Reticulocytes
- C. Heinz bodies
- D. May-Hegglin anomaly
Correct Answer: C 🗳️
True Statements:
Urine should be well mixed prior to dipping the reagent strip. Prolonged immersion may wash out test reagents.
False Statements:
Urine should be centrifuged prior to dipping the reagent strip. When visually reading the reagent strip, all results can be read immediately after dipping the strip in the urine specimen.
Which of the following statements are TRUE regarding the reagent strip test procedure? (Choose ALL of the correct answers)
- A. Prolonged immersion may wash out test reagents.
- B. Urine should be centrifuged prior to dipping the reagent strip.
- C. When visually reading the reagent strip, all results can be read immediately after dipping the strip in the urine specimen.
- D. Urine should be well mixed prior to dipping the reagent strip.
Correct Answer: A,D 🗳️
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